First Report of Brown Rot Caused by <i>Monilinia polystroma</i> on Apple in Italy

HomePlant DiseaseVol. 105, No. 11First Report of Brown Rot Caused by Monilinia polystroma on Apple in Italy PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ItalyM. Rosati, G. Schiavon, M. L. Gullino, and D. SpadaroM. RosatiCentre Competence for the Innovation Agro-environmental Sector (AGROINNOVA), University Turin, I-10095 Grugliasco, TO, ItalyDepartment Agricultural, Forestry Food Sciences, Torino, ItalySearch more papers this author, SchiavonCentre Gullinohttps://orcid.org/0000-0002-7706-1915Centre Spadaro†Corresponding author: Spadaro; E-mail Address: [email protected]://orcid.org/0000-0001-5207-9345Centre author AffiliationsAuthors Affiliations Rosati1 2 Schiavon1 Gullino1 Spadaro1 † 1Centre 2Department Published Online:24 Nov 2021https://doi.org/10.1094/PDIS-03-21-0548-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBrown rot is a common apple disease Italy, caused fructicola, laxa, fructigena (Martini et al. 2013). In September 2020, ‘Jeromine’ orchard under integrated pest management located Scarnafigi (44°39′N, 7°33′E, north-western Italy), fruits (8.6%) showing brown blackish firm lesions (6.0 8.0 cm diameter) were observed. some fruits, rots covered yellowish stromata. Two isolates (MPI1, MPI2) obtained from two symptomatic apples cultured potato dextrose agar (PDA) 7 days at 25°C 12-h light/12-h dark regime. A white-to-greyish mycelium with slightly undulate margins irregular, black stromata developed PDA after 12 incubation. Conidia, observed branched monilioid chains, one-celled, globose, limoniform, hyaline, 38 58 (mean: 48) × 20 44 33) μm. Based morphology, tentatively identified as (G.C.M. Leeuwen) Kohn. polymerase chain reaction primers ITS1 ITS4 was performed internal transcribed spacer (ITS) regions 1 5.8S gene. The sequenced amplicons (435 445 bp; GenBank accession nos. MW600854 MW600855) showed 100% identity reference isolate (HQ846944) other (AM937114, JX315717) plum (GU067539). ITS region had five nucleotides distinguish it closest species, (Zhu 2016; MH862738). pathogenicity both tested mature Jeromine (10.1% total soluble solids). Three replicates six per surface disinfected 1% NaClO. mycelial plug (5 mm) colony grown inserted using cork borer into hole (6 each fruit (Vasić 2016). Apples inoculated sterile plugs used control. Fruits placed 22 ± 1°C, 85% relative humidity, Lesion size measured 3, 6, 9 All typical symptoms 6 inoculation, appeared surface; control remained healthy. virulence statistically similar. reisolated all confirmed molecular methods. This first report Italy. previously reported Hungary (Petróczy 2009), apricot Switzerland (Hilber-Bodmer 2012), peach pear 2014, 2015), China 2016), Serbia 2018). emergence pathogen pome stone production Europe stimulates study its biology epidemiology, fitness management, compared endemic species.The author(s) declare no conflict interest.References:Hilber-Bodmer, M., 2012. Plant Dis. 96:146. https://doi.org/10.1094/PDIS-06-11-0522 Link, ISI, Google ScholarMartini, C., 2013. 97:689. https://doi.org/10.1094/PDIS-09-12-0869-PDN 2014. 98:1585. https://doi.org/10.1094/PDIS-05-14-0551-PDN 2015. 99:556. https://doi.org/10.1094/PDIS-10-14-1006-PDN ScholarPetróczy, 2009. Eur. J. Pathol. 125:343. https://doi.org/10.1007/s10658-009-9476-5 Crossref, ScholarVasić, 2016. 144:15. https://doi.org/10.1007/s10658-015-0740-6 2018. 102:359. https://doi.org/10.1094/PDIS-06-17-0867-RE ScholarZhu, X. Q., 100:2240. https://doi.org/10.1094/PDIS-03-16-0325-RE ScholarFunding: Funding provided Fondazione Cassa Di Risparmio di Cuneo (SMART APPLE - Innovative SMART technologies).The interest.DetailsFiguresLiterature CitedRelated Vol. 11 November 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionFoliar mottling interveinal yellowing melon leaves Central Valley California (S. Mondal al.). Photo credit: W. Wintermantel. Improved wheat plant growth Pratylenchus neglectus infested soil applying aldicarb time planting (R. Smiley R. Smiley. Mosaic feathery mottle taro foliage dasheen mosaic virus (C. Escalante A. Valverde. Metrics Downloaded 531 times Article History Issue Date: Jan 2022Published: 24 2021First Look: Jun 2021Accepted: 3 2021 Page: 3761 Information© American Phytopathological SocietyFundingFondazione CuneoGrant/Award Number: technologiesKeywordsbrown rotappleMonilinia polystromaItalyThe interest.PDF download